From understanding CRISPR biology to CRISPR 2.0 applications
The CRISPR immune system protects bacteria and archaea from invading viruses and plasmids. Immunity depends on protein complexes that use small RNA molecules to find matching viral or plasmid DNA. I will show how viruses escape immunity by mutating their DNA, and how a mechanism called priming takes care of these escaped viruses and will quickly update the memory of the immune system. All of these insights into the biology of CRISPR has led to some of the most revolutionary molecular genetics tools to date, with Cas9 being the most well known example. I will highlight some new CRISPR tools for genome engineering approaches to edit genomes and to knockdown gene expression.
Stan J.J. Brouns
Department of Bionanoscience, Kavli institute of Nanoscience, Delft University of Technology, Netherlands
Laboratory of Microbiology, Wageningen University, Netherlands