In the last few years the Re-scan Confocal Microscope has become a well-known and simple technology to obtain images with an improved resolution (170 nm) as compared to standard confocal microscopy (240 nm). The technology is based on a standard confocal microscope with an additional scanner (the re-scanner) that directs the emitted light to a sensitive (CMOS or CCD) camera. Precise control of the scanner (that “reads” the sample) and re-scanner (that “writes” the image on the camera) allows super-resolution imaging without closing the pinhole to a minimum. This highly photon-economical way of detection (no losses at the pinhole) and the use of a highly sensitive camera reduce the noise level in RCM images. Apart from imaging with better resolution and less noise the RCM microscope has a lot more potential.
In the last few years we have exploited the possibilities of the RCM technology. We have adjusted the microscope for different fluorescence techniques and modalities. In this presentation we will discuss the new technical developments we are working on.
Confocal.nl was founded in 2016 by Peter Drent and Erik Manders and is a spin-off of the University of Amsterdam. Confocal.nl successfully brings the Re-scan Confocal Microscope (RCM) to the international market. In the future new technological alterations and improvements of RCM will be added to the list of products. Confocal.nl is always searching for people with enthusiasm and clever ideas to work on the RCM technology.